DensitoQuant is an easy-to-use, fast and accurate, stain-intensity-based IHC quantification tool. The application identifies the positive stain, based on an automatic color separation method through which individual positive pixels are counted and classified based on intensity and threshold ranges. This way, an H-Score can be calculated based on the proportion of positive and negative pixels.
Molecular Pathology
Fluorescent in situ hybridization (FISH) and Chromogenic in situ hybridization (CISH) are routinely used methods in histopathological diagnostics. The visual evaluation of these samples is a difficult task, and typically a time-consuming process and prone to human errors that can be eliminated by using image analysis solutions.