Recombinant antigen reagent RP00002

for researchapoptosislyophilized

Active Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus. Bio-Activity - 1.Recombinant Human IL1β protein was measured by NF-κB reporter assay in HEK293 cells. The ED50 for this effect is 1-10 pg/mL. 2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.217-0.870 ng/mL. 3.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL. Background Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Usage guide Reconstitution - Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.