Recombinant antigen reagent RP01262

for researchlyophilizedSARS-COV-2

Recombinant SARS-COV-2(2019-nCoV) Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of SARS-COV-2(2019-nCoV) Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus. Bio-Activity - Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1 at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.|Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay (Nicoya OpenSPR). Endotoxin - < 1.0 EU/μg of the protein by LAL method. Formulation - Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation. Calculated MW - 75.8kDa Background The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity. Product information Synonym - S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein Expressed Host - HEK293 cells Tag - 6×His tag at the C-terminus Gene ID - 43740568