Monoclonal antibody A19611

for Western blotimmunohistochemistryanimal-based

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017] Immunogen information Immunogen - A synthesized peptide derived from human Androgen Receptor. Sequence - Gene ID - 367 Swiss prot - P10275 Synonyms - AIS; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM; AR Calculated MW - 110kDa Observed MW - 110KDa Applications Reactivity - Human, Mouse Tested applications - WB IHC IF Recommended dilution - WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 Storage buffer - Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. Application key - Western blotting Immunohistochemistry Immunofluorescence Positive samples - Mouse testis Cellular location - Cytoplasm, Nucleus