Enzyme reagent RK21103

for microbiologypHglycerol

One unit is defined as the amount of enzyme required to digest 1 µg of pBC4 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions - 10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 0.15% Triton X-100, pH 7.4 @ 25°C Reaction Conditions - 1X Buffer CutS, Incubate at 37°C. Reaction Buffer or Mix - 1X Buffer CutS: 50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C Heat Inactivation - 65°C for 20 min Methylation Sensitivity - dam methylation - Not Sensitive dcm methylation - Not Sensitive CpG Methylation - Blocked