T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.
Product Features
Application: It can catalyze the formation of phosphodilipid bond at flat end and sticky end, and repair double-stranded DNA, RNA, DNA/RNA hybrid strand cutting;
Suitable for various types of samples: gDNA/FFPE DNA/cfDNA/ChIP DNA;
Suitable for many types of segment connection: compatible with Illumina platform Y connector and MGI platform Bubble connector segment;
Strict quality control: strict batch performance and stability quality control;
Applications
Cloning Ligation