Solution reagent kit MOLgen

for RT-PCRrespiratory syncytial virusRSV

Acute respiratory infections are common respiratory diseases that generally show minor symptoms but depending on the patient’s condition can also cause morbidity, hospitalization and death. The pathogens that are the main infectious agents of these respiratory infections are FLU A, FLU B, RSV A, RSV B, and SARS-CoV-2. The nature of respiratory transmission of this category of viruses has the potential for wide-ranging spread and is a major concern of both local and international public health levels. Given that these pathogens cause respiratory syndromes with similar symptoms, it is necessary to distinguish them especially in relation to the pandemic caused by SARS-CoV-2. Principle Of Method Respiratory Panel (RT-5) is a multiplex real-time one step RT-PCR assay for the detection of Influenza A, Influenza B, Human Respiratory Syncytial Virus A, Human Respiratory Syncytial Virus B, SARS-CoV-2 using real time polymerase chain reaction (RT-PCR) method to quickly detect types of respiratory viruses via fluorescence detection of amplified targets. During the PCR process, if the NA of this viruses is amplified during each cycle, a hydrolysis probe method is used to determine whether genes are amplified by fluorescence of this amplified products. The detection of amplified targets fragment is performed on channel FAM (FLU), ROX/Texas Red (SARS-CoV-2), and CY5 (RSV). The use of human β-actin as Internal Control (IC) on Cy5.5/Alexa Fluor channel ensures the correct monitoring of the cellularity of the samples.