Tuberculosis assay kit MOLgen

mycobacterium tuberculosis complexclinicalblood

“Molgen DNA MBTC Kit” is an assay kit for the detection of Mycobacterium tuberculosiscomplex DNA by Real-Time PCR method. Introduction “Molgen DNA MBTC Kit” is intended for the detection of DNA of the Mycobacterium tuberculosis complex (containing mycobacteria causing tuberculosis in humans: M. tuberculosis, M. bovis, M. bovis BCG, M. microti, M. Africanum) extracted from clinical specimens using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. “Molgen DNA MBTC Kit” is designed for use with block cyclers iQ5 iCycler, CFX96 (Bio-Rad, USA), DT96 (DNA-Technology, Russia). The assay kit may be used in clinical practice for testing clinical materials (blood plasma, biopsy materials, cerebrospinal fluid, urine, bronchopulmonary lavage, sputum, synovial fluid). The extraction of DNA can be performed using the extraction kit of MOLgen series (manufactured by Adaltis). When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. “Molgen DNA MBTC Kit” contains reagents required for 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.