Dye reagent kit MOLgen

clinicalfor RNA detectionHAV

MOLgen RNA HAV1 S1 Kit is intended for detection of hepatitis A virus (HAV) RNA by real-time RT-PCR. The kit contains reagents for real-time RT-PCR only. Introduction “Molgen RNA HAV S1 Kit” is intended for the detection of hepatitis A virus (HAV) RNA in clinical specimens (blood serum and plasma, faeces) and water specimens using the method of reverse transcription of viral RNA with subsequent cDNA amplification by real-time polymerase chain reaction (RT-PCR) with fluorescence detection of amplified product. The extraction of RNA from clinical specimens can be performed using “MOLgen Universal Extraction Kit” (manufactured by Adaltis S.r.l.). When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. The results of RT-PCR analysis are taken into account in complex diagnostics of disease. Set 1 is intended for use with block-type PCR cyclers: AMPLIlab (Adaltis), iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 48 tests, including control samples. NOTE: The extraction protocol and the PCR set-up procedure can be done in automated mode using the Adaltis EXTRAlab Instrument by executing the extraction and PCR set-up protocol preloaded in the instrument user interface. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogen’s cDNA. Fluorescence signal increases due to the separation of fluorescence dye