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Sexually transmitted disease assay kit MOLgen
Mycoplasma genitaliumurinesaliva

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Characteristics

Applications: for sexually transmitted diseases
Micro-organism: Mycoplasma genitalium
Sample type: clinical, urine, saliva
Analysis mode: fluorescence

Description

“Molgen DNA Mycoplasma genitalium S1 Kit” is an assay kit for the detection of Mycoplasma genitalium DNA by real-time PCR. Introduction “Molgen DNA Mycoplasma genitalium S1 Kit” is intended for the detection of Mycoplasma genitalium DNA in clinical specimens (saliva, urine, swabs of the epithelial cells) using real-time polymerase chain reaction (PCR) method with fluorescent detection of amplified product. The kit is validated for use with block-type PCR cyclers: iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). The extraction of DNA can be performed using the“Molgen Universal Extraction Kit”. When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. The kit contains reagents requiredfor 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence

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Sexually transmitted disease assay kit MOLgenMycoplasma genitaliumurinesaliva

Characteristics

Description

Catalogs

Other ADALTIS products

Molecular Diagnostic Tests

Sexually transmitted disease assay kit MOLgen
Mycoplasma genitaliumurinesaliva