“MOLgen DNA Candida albicans / Gardnerella vaginalis S1 Kit” is an assay kit for the detection of Candida albicans and Gardnerella vaginalis DNAby Real-Time PCR method.
Introduction
“MOLgen DNA Candida albicans / Gardnerella vaginalis S1 Kit” is intended for the detection of Candida albicans and Gardnerella vaginalis DNA extracted from clinical specimens (epithelial cell swabs, urine, prostate fluid, semen) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product.
The extraction of Candida albicans and Gardnerella vaginalis DNA from clinical specimens (epithelial cell swabs, urine, prostate fluid, semen) can be performed using the “Molgen Universal Extraction Kit”.
The results of PCR analysis are taken into account in complex diagnostics of disease
Set 1 is intended for use with block-type PCR cyclers iQ5 iCycler, CFX96 (Bio-Rad, USA), DT96 (DNA-Technology, Russia).
Set 1 contains reagents required for 96 tests, including control samples.
Principle Of Method
Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.
Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold