DNA extraction assay kit MOLgen

for Epstein-Barr virussaliva

“Molgen DNA EBV S1 Kit” is an assay kit for the detection of Epstein-Barr Virus DNA by Real-Time PCR method. Introduction Pathogen information EBV is a herpesvirus that infects the vast majority of the world's adult population. Comparatively large (172 kilobase pairs) EBV genome contains about 85 genes not only involved in direct viral replication but also helping to avoid host immune response and control cell cycle. Following primary infection, EBV persists in the infected host as a lifelong asymptomatic infection. Although EBV can infect different cell types, it is B-cells and epithelial cells in the oropharynx that maintain a major part of viral replication for a long period. Some of infected B-cells become memory cells where EBV stays latent indefinitely. Latent EBV in B-cells can be reactivated and switch to lytic replication [1]. The virus can be found in many types of bodily fluids of infected person but commonly is transmitted with saliva. Primary infection in childhood is usually asymptomatic or cause nonspecific mild respiratory disease. However, in western countries with affluent populations, where exposure is generally delayed until adolescence or young adulthood, primary infection is commonly associated with infectious mononucleosis (IM). Over 50 percent of patients with IM manifest the triad of fever, lymphadenopathy, and pharyngitis; splenomegaly, palatal petechiae, and hepatomegaly are each present in >10 percent of patients. Less common complications include hemolytic anemia, thrombocytopenia, aplastic anemia, myocarditis, hepatitis, genital ulcers, splenic rupture, rash,