Herpes assay kit MOLgen

herpes simplex virusurinesaliva

“MOLgen DNA HSV-1 / HSV-2 S1 Kit” is an Assay Kit for differential determination of Herpes Simplex Virus Types 1 and 2 DNA by Real-Time PCR method. Introduction “MOLgen DNA HSV-1 / HSV-2 S1 Kit”is designed for differential determination of herpes simplex virus types 1 and 2 DNA isolated from clinical specimens using extraction kit: “MOLgen Universal Extraction Kit”. Assay is based on real-time polymerase chain reaction (PCR) method with fluorescent detection of amplified product. The kit contains reagents requiredfor 96 tests, including control samples. The kit is designed for use with iQ5 iCycler (Bio-Rad, USA), CFX96 (Bio-Rad, USA) and DT-96 (DNA-Technology Research and Production Company, Russia). “MOLgen DNA HSV-1 / HSV-2 S1 Kit” is designed for the analysis of clinical materials (scrapings of the epithelial cells, saliva, semen, prostatic fluid, urine, serum, plasma, leukocytic mass, whole blood). Principle Of Method Real-time PCR is based on the detection of fluorescence, produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is dual-labeled DNA-probe, which specifically binds to the target region of pathogen DNA. Fluorescent signal increases due to the fluorescent dye and quencher separating by Taq DNA-polymerase exonuclease activity during amplification. PCR process consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value - Ct – is the cycle number at which the fluorescence generated within a reaction crosses the fluorescence threshold, a fluorescent signal rises significantly above the background fluorescence.