TORCH infection assay kit MOLgen

for DNAparvovirusblood

“Molgen DNA Parvovirus B19 S1 Kit” is an assay kit for the detection of Parvovirus B19 DNA by real-time PCR method. Introduction “Molgen DNA Parvovirus B19 S1 Kit” is intended for the detection of Parvovirus B19 DNA in clinical specimens (blood serum, plasma, saliva) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. The extraction of DNA from clinical specimens can be performed using “Molgen Universal Extraction Kit”. When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. The results of PCR analysis are taken into account in complex diagnostics of disease. Set 1 is intended for use with block-type PCR cyclers: AMPLIlab (Adaltis), iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogen’s DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher owing to Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background.