TORCH infection assay kit MOLgen

varicella-zoster virusbloodurine

“Molgen DNA VZV S1 Kit” is an assay kit for the detection of Varicella-zoster virus DNA by real-time PCR. Introduction “Molgen DNA VZV S1 Kit” is intended for the detection of Varicella-zoster virus DNA using the method of real-time polymerase chain reaction (PCR) with fluorescent detection of amplified product. Set 1 is intended for use with iQ5 iCycler (Bio-Rad, USA), CFX96 (Bio-Rad, USA) and DT-96 (DNA-Technology, Russia). The extraction of DNA from the clinical specimens (scrapings of epithelial cells and tissue fluids of erosive-ulcerative skin lesion and human mucosa, urine, saliva, blood serum (plasma) can be performed using the“Molgen Universal Extraction Kit”. Set 1 contains reagents requiredfor 48 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence: it binds to one of the DNA strands in the course of reaction