DNA extraction assay kit MOLgen

EhrlichiaAnaplasma phagocytophilumblood

“MOLgen DNA Anaplasma phagocytophilum / Ehrlichia muris / Ehrlichia chaffeensis S1 Kit” is an assay kit for the detection of Anaplasma phagocytophilum,Ehrlichia muris and Ehrlichia chaffeensis DNA by real-time PCR. Introduction “MOLgen DNA Anaplasma phagocytophilum / Ehrlichia muris / Ehrlichia chaffeensis S1 Kit”is intended to detect DNA of Anaplasma phagocytophilum(causative agent of human granulocytic anaplasmosis (HGA)), Ehrlichia muris and Ehrlichia chaffeensis (pathogens causing human monocytotropic ehrlichiosis (HME)) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. The results of PCR analysis are taken into account in complex diagnostics of disease. The extraction of DNA from clinical specimens (blood serum, plasma, leukocyte blood fraction, tick suspensions) can be performed using“Molgen Universal Extraction Kit”. When using DNA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. Set 1 is intended for use with block-type PCR cyclers: iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 48 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification.