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Molecular diagnostic assay kit ME184470
HPVclinicalfluorescence

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Characteristics

Applications: for molecular diagnostic
Micro-organism: HPV
Sample type: clinical
Analysis mode: for real-time PCR, fluorescence

Description

Reagent kit is intended for detection of Human papilloma virus (HPV) type 44 DNA in clinical specimens by real-time PCR. The kit contains reagents for real-time PCR only. Introduction “MOLgen DNA HPV 44 S1 Kit” is intended for the detection of human papillomavirus (HPV)type 44 DNA, in clinical specimens (epithelial cells swabs) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. The extraction of DNA from clinical specimens can be performed using the “Molgen Universal Extraction Kit”.When using NA extraction kits of other manufacturers, it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis S.r.l. The results of PCR analysis are taken into account in complex diagnostics of disease. Set 1 can be used for quantitative determination of HPV type 44 DNA when using the kit in set with “MOLgen DNA HPV HCR Genotype (quantitative) S1 Kit”. Set 1 is intended for use with block-type PCR cyclers:AMPLIlab (Adaltis), iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.

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Molecular diagnostic assay kit ME184470HPVclinicalfluorescence

Characteristics

Description

Catalogs

Other ADALTIS products

Molecular Diagnostic Tests

Molecular diagnostic assay kit ME184470
HPVclinicalfluorescence