Molecular diagnostic assay kit ME184750

HPVclinicalfluorescence

“Molgen DNA HPV 6/11 S1 Kit” is an assay kit for the differential determination of Human Papillomavirus types 6 and 11 DNA by real-time PCR. Introduction “Molgen DNA HPV 6/11 S1 Kit” is intended for the differential determination of DNA of Human Papillomavirus (HPV) types 6 and 11 using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. Set 1 is intended for use with block-type PCR cyclers: iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). The assay kit may be used in clinical practice for testing clinical material. The extraction of Human Papillomavirus (HPV) types 6 and 11 DNA from clinical specimens can be performed using the “Molgen Universal Extraction Kit”. Set 1 contains reagents requiredfor 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence