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Molecular diagnostic assay kit ME184460
HPVclinicalfluorescence

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Characteristics

Applications: for molecular diagnostic
Micro-organism: HPV
Sample type: clinical
Analysis mode: for real-time PCR, fluorescence

Description

Reagent kit is intended for detection of Human papilloma virus (HPV) types 6, 11 and 44 DNA in clinical specimens by real-time PCR. The kits contain reagents for real-time PCR only. Introduction “MOLgen DNA HPV 6/11/44 S1 Kit” is intended for the detection of DNA of human papillomavirus (HPV) types 6, 11 and 44 DNA in clinical specimens (epithelial cells swabs) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. The extraction of DNA from clinical specimens can be performed using the “Molgen Universal Extraction Kit”.When using NA extraction kits of other manufacturers, it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis S.r.l. The results of PCR analysis are taken into account in complex diagnostics of disease. Set 1 is intended for use with block-type PCR cyclers:AMPLIlab (Adaltis), iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold

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Molecular diagnostic assay kit ME184460HPVclinicalfluorescence

Characteristics

Description

Catalogs

Other ADALTIS products

Molecular Diagnostic Tests

Molecular diagnostic assay kit ME184460
HPVclinicalfluorescence