Bacterial vaginosis assay kit MOLgen

Mycoplasmaurinesaliva

“MOLgen DNA Mycoplasma hominis S1 Kit” is an assay kit for the detection of Mycoplasma hominis DNAby Real-Time PCR method. Introduction “MOLgen DNA Mycoplasma hominis S1 Kit” is designed to detect Mycoplasma hominis DNA isolated from clinical specimens using “MOLgen Universal Extraction Kit”. Assay is based on real-time polymerase chain reaction (PCR) method with fluorescent detection of amplified product. The kit contains reagents requiredfor 96 tests, including control samples. The kit is validated for use with iQ5 iCycler (Bio-Rad, USA), CFX96 (Bio-Rad, USA) and DT-96 (DNA-Technology, Russia). “MOLgen DNA Mycoplasma hominis S1 Kit” is designed for the analysis of clinical materials (saliva, urine, scrapings of the epithelial cells). Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence: it binds to one of the DNA strands in the course of reaction and provides additional specificity of the method.