Molecular diagnostic assay kit MOLgen

Candida albicansurineclinical

“MOLgen DNA Candida albicans S1 Kit” is an assay kit for the detection of Candida albicans DNAby Real-Time PCR method. Introduction “MOLgen DNA Candida albicans S1 Kit” is intended for the detection of Candida albicans DNA, extracted from clinical specimens (epithelial cells swabs, semen, prostatic fluid, urine), using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. Set 1 is intended for use with block-type PCR cyclers: iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). The extraction of DNA can be performed using the“Molgen Universal Extraction Kit”. When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. Set 1 contains reagents requiredfor 96 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence