Molecular diagnostic assay kit MOLgen

geneticwhole bloodclinical

“MOLgen Genetics AZF-microdeletions Kit” is an assay kit for the differentialdetermination of Y-chromosome AZF locus deletions using real-time PCR. Introduction “MOLgen Genetics AZF-microdeletions Kit” is intended for the differential determination of AZF locus deletions on the human Y-chromosome in clinical specimens (whole blood, buccal epithelium) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. The extraction of DNA from clinical specimens can be performed usingthe “MOLgen Universal Extraction Kit”manufactured by Adaltis S.r.l. The results of PCR analysis are taken into account in complex diagnostics of disease. The kitis intended for use with block-type PCR cyclers: AMPLIlab (Adaltis), CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). The kit contains reagents required for 48 tests, including control samples. NOTE: The extraction protocol and the PCR set-up procedure can be done in automated mode using the Adaltis EXTRAlab Instrument by executing the extraction and PCR set-up protocol preloaded in the instrument user interface. Principle Of Method AZF microdeletions occur in the Y-chromosome regions responsible for spermatogenesis causing abnormalitiesin spermatogenesis and, therefore, male infertility. At molecular level, deletions extend over 0.8 - 7.7 Mb, including several genes, but the size of AZF loci is too small to be detected by cytogenetics methods, that is why “microdeletions” term is used. According to “EAA/EMQN best practice guidance for molecular diagnosis of Y-chromosomal microdeletions”,