Optimized Buffers were specifically developed to provide highly reproducible retention times with HPLC System Instrumentation. Optimized Buffers have a longer shelf life than buffers prepared from concentrate. They will allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems.
Optimized Buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically leads to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. Optimized Buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
Measurements are done gravimetrically to ensure correct concentrations of TEAA and acetonitrile
Manufactured with the purest chemicals in a metal-free and clean environment
Bottles are conveniently shaped and sized allowing the neck of the Optimized Buffer bottles to fit exactly into the HPLC system cap
Highly reproducible retention times from batch to batch