❖ Rapid detection of the Novel SARS-CoV-2 (Covid-19) in around 1 hour
❖ No additional specialist instrumentation; testing carried out on standard qPCR machines
❖ Low sample volume requirements
❖ Internal positive control to confirm RNA quality is acceptable for diagnostic testing
❖ Stable at -20 °C for up to 18 months
Overview:
The novel SARS-CoV-2 (COVID-19) is a severe acute respiratory syndrome coronavirus which is responsible for respiratory disease was first detected in Wuhan City, Hubei Province, China, later causing a global pandemic. The virus is contagious and is spread primarily through the exchange of mucus droplets that are expelled through sneezes or coughs.
This outbreak is an important reminder that the global community must strengthen national and international programs for detection and response to future disease outbreaks.
Product Details:
Zena Max SARS-CoV-2 Direct qPCR assay system is a RealTime PCR that enables the direct amplification of COVID-19 infection in human samples like nasopharyngeal swabs (NPS), nasal swabs, nasal wash or aspirate or bronchoalveolar lavage (BAL) using one-step RT-qPCR by first reverse transcribing the genomic RNA target to cDNA, followed by amplification of the assay target and detection by the hydrolysis probe method of qPCR. The assay consists of a forward primer, a reverse primer, and a probe labelled with the 5’ FAM™ and ROX reporter dye with a 3’ quencher. As the new target cDNA strand is synthesised, the tightly bound probe is cleaved by the 5’ to 3’ exonuclease activity of Taq polymerase which releases the fluorescent reporter from the quencher and substantially increases the fluorescent signal.