The EliSpot technology is widely used to quantify antigen-specific cellular immunity on a single-cell level.
The T cell reaction against a specific antigen is the central part of the cellular immune answer. The individual immunity can be monitored in detail based on the variability in correlation with the number of activated T cells, respectively the type of secreted cytokine.
The EliSpot assay is a well standardized method and easily automatable for different issues and questions and is therefore used in research as well as in diagnostic laboratories.
Characteristics
High sensitivity compared to comparable systems like ELISA
Results more meaningful by the detection of functional T cells
Universal platform for combination of different available antigens and manifold issues