Solution reagent kit OPAL 780

stainantibodypathology

Opal™ is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed. Standalone Opal Fluorophore reagent packs allow you to pick and choose your panel's biomarker quantity and design. The Opal 780 Reagent Pack is a two-part reaction and part of the Phenoptics 2.0 7-color whole slide workflow. The Reagent Pack contains: Opal TSA-DIG, 1 vial Opal Polaris 780, 1 vial Dimethyl sulfoxide (DMSO), 1 x 100 uL Users should source primary antibodies and other workflow reagents separately. Pack contains enough reagent to stain up to 50-slides. Generally, 100-300 µL of Opal Working Solution is required per slide. Protocol for Opal TSA-DIG reconstitution and dilution: Dissolve the Opal TSA-DIG Reagent in 75 uL of DMSO. Before each procedure, dilute Opal TSA-DIG in 1X Plus Amplification Diluent for manual staining or 1X Plus Automation Amplification Diluent for automation to make Opal TSA-DIG Working Solution. The recommended dilution ratio is 1:100. Protocol for Opal 780 reconstitution and dilution: Dissolve the Opal 780 Reagent in 300 uL of ddH2O. Before each procedure, dilute Opal 780 in Blocking/Ab Diluent to make Opal 780 Working Solution. The recommended dilution ratio is 1:25. The Opal Reagent Packs can be utilized for both mono- and multiplex tissue staining and have been configured and validated for imaging on the PhenoImager HT (Formerly Vectra® Polaris™) quantitative pathology imaging system.