The cAMP Complete ELISA is a colorimetric competitive immunoassay for the quantitative determination of extracellular cAMP in cell lysates, culture supernatants, serum, saliva, and tissue.
Reagents included
Sensitivity is increased 10-fold by acetylation
Absorbance is read at 405 nm
Large signal-to-background ratio offers superior sensitivity
Easily assess adenylyl cyclase activation by GPCRs
Product Distribution
Available Worldwide
Range
See Package Insert
Sensitivity
See Package Insert
Sizes
96 Wells
Additional Information
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution.