Research test kit 30-VELHU-E01

serumplasmaELISA

The Vedolizumab Drug Level ELISA is intended for the quantitative determination of free Entyvio® concentration in human serum and EDTA plasma. Research Use Only. Not for Use in Diagnostic Procedures. Specifications Regulatory Status Research Use Only. Not for Use in Diagnostic Procedures. Product Distribution Available in North America Only Range 15-1000 ng/mL Sensitivity 15 ng/mL Sizes 96 Wells Inc Time Minute 20 Inc Time Overnight No Inc Time See Protocol No Sample Size 100 Additional Information This ELISA is designed to quantitate free vedolizumab (therapeutic antibody against α4β7 integrin, e. g. Entyvio®) in EDTA plasma or serum samples. In a first incubation step, the free vedolizumab from the sample is bound to the specific monoclonal anti-vedolizumab antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, Peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free vedolizumab in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standard. The concentrations of free vedolizumab in the samples are determined directly from this curve.