10x Combination Buffer is a balanced ammonium-potassium PCR buffer. Combination Buffer results in tolerance towards optimization of primer annealing temperatures and Mg2+ concentrations.
FEATURES
Promotes reliable amplification on most DNA templates
Tolerance toward primer annealing temperature
10x Buffer
AVAILABLE IN 4 FORMULATIONS:
15 mM MgCl2,
Mg2+ free
Tween free
Mg2+ free + Tween free
DESCRIPTION
10x Ammonium Buffer is recommended for most PCR applications however; we recommend you to use 10x Combination Buffer as it show good results on certain PCR instruments. 10x Combination Buffer is also worth testing when searching for PCR buffers to be used in new set-up.
TIP - CHOOSE THE RIGHT BUFFER
10x Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.