Buffer solution reagent A302 series

for PCRliquid

Standard Buffer is the traditional potassium buffer and promotes high specificity. FEATURES Promotes reliable amplification on most DNA templates High specificity 10x Buffer AVAILABLE IN 4 FORMULATIONS: 15 mM MgCl2, Mg2+ free Triton-X 100 free Mg2++ Triton-X 100 free DESCRIPTION Ammonium Buffer is recommended for most PCR applications however; we recommend that you continue using Standard Buffer if you have already optimised your protocols for this buffer. The use of Standard buffer most often requires optimisation of primer annealing temperatures and Mg2+ concentrations. Highly pure DNA templates are preferable if you use this buffer. TIP - CHOOSE THE RIGHT BUFFER Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures. Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM. Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.