Solution reagent PureIT

for PCRNGSfor DNA cleanup

One-step PCR Clean-up for optimal sequencing results. Clean-up results in 5 minutes. FEATURES One-step PCR product clean up Fast 5 min protocol No Sample Loss No need for spin columns or magnetic beads Treatment improves downstream applications such as DNA sequencing and SNP analysis Ideal for NGS library preparation protocols DESCRIPTION PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP). Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for minimum 2 minutes, PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for minimum 3 minutes.