DNA polymerase reagent RealQ Plus

dyedNTP solutionfor real-time PCR

RealQ Plus Master Mix Green enables real-time-based DNA amplification with high specificity and efficiency. Real-time PCR is a sensitive and reliable method for gene analysis and DNA quantification. RealQ Plus 2x Master Mix Green is the right choice, when expenses and experiment preparation time might be limited or if you need to quickly analyse many genes. FEATURES Reliable master mix for real-time PCR Based on DNA-binding fluorescent dye detection Reliable quantification High specificity, stability, reproducibility and efficiency Reaction set-up at room temperature Premixed all-in-one 2x solution DESCRIPTION RealQ Plus 2x Master Mix Green is composed of TEMPase Hot Start DNA Polymerase, dNTPs, fluorescent dye and an optimised buffer system. Furthermore, this master mix is available with high, low or without ROXTM ensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template and primers. When the fluorescent Green dye is free in the solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA.