Ammonium buffer reagent A301 series

for PCRliquid

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures. FEATURES Promotes amplification on most DNA templates High yield High specificity Tolerance for primer annealing temperature 10x Buffer AVAILABLE IN 4 FORMULATIONS: 15 mM MgCl2, Mg2+ free Tween free Mg2+ + Tween free DESCRIPTION Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences. TIP - CHOOSE THE RIGHT BUFFER Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM. Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.