2X PCR Master Mix is an optimized ready-to-use PCR mixture of Recombinant Taq DNA Polymerase, dNTPs, and PCR Buffer including optimal concentration of MgCl2. 2X PCR Master Mix contains all necessary components for PCR, except template DNA and primers. Generated PCR products are compatible with all downstream applications such as sequencing and cloning.
For research use only.
1 ml vial contains 250 units of Taq DNA polymerase
Sufficient for 100 reactions at 10 μl master mix per reaction (i.e., 20 μl total reaction volume)
Compatible with T/A cloning (generates 3′ dA overhangs)
Compatible with standard PCR, DNA labeling, DNA sequencing, and other applications for a thermostable DNA polymerase
May be shipped or temporarily stored at room temperature.
TECHNICAL SPECIFICATIONS
Contents:
0.25 U/μl Taq DNA polymerase
2X PCR buffer
0.4 mM dNTPs (dATP, dCTP, dGTP and dTTP)
3.2 mM MgCl2
May be shipped at room temperature without discernible loss of activity