Bosphore HIV-1 Quantification Kit detects human immunodeficiency virus 1 RNA in human serum and plasma, encompassing all the HIV-1 genotypes. A region within LTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition and extraction. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or PCR step.