Tropical disease test kit Bosphore®

virusWest Nile virusSalmonella

Bosphore Tropical Fever Panel Kit v1 detects and characterizes DNA/RNA of Plasmodium falciparum, Plasmodium malariae, Salmonella enterica, Plasmodium vivax, dengue virus, chikungunya virus, West Nile virus, Plasmodium ovale and Leptospira spp. in human biological samples such as whole blood, plasma, serum, ocular fluids and urine. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters. In the first tube, Plasmodium falciparum is amplified and fluorescence detection is accomplished using the FAM filter, Plasmodium malariae is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella enterica is amplified and fluorescence detection is accomplished using the Cy5 filter. In the second tube, Plasmodium vivax is amplified and fluorescence detection is accomplished using the FAM filter, dengue virus is amplified and fluorescence detection is accomplished using the HEX filter and chikungunya virus is amplified and fluorescence detection is accomplished using the Cy5 filter with PCR Master Mix 2. In the third tube, West Nile virus is amplified and fluorescence detection is accomplished using the FAM filter, Plasmodium ovale is amplified and fluorescence detection is accomplished using the HEX filter and Leptospira is amplified and fluorescence detection is accomplished using the Cy5 filter with PCR Master Mix 3. An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.