Reverse transcriptase reagent

magnetic bead-basedRT-qPCRlyophilized

Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler). Description Shelf life - 12 months Concentration - Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction Description - Applyo RT-qPCR Beads contain all reagents for detection of RNA (and DNA) in a lyophilized bead format. This includes among others a polymerase and reverse transcriptase and buffer components for performing a RT-qPCR reaction. User only needs to add target specific primers and probes. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. Handling Applyo RT-qPCR Beads Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. Recommended RT-qPCR protocol for Applyo RT-qPCR Beads Handling of Applyo Beads