Solution reagent BeaverBeads™ Magrose Strep-Tactin

magnetic bead-basedfor protein purification

The Strep Tactin beads based on superparamagnetic beads are designed for rapid and efficient separation Strep（II）-tag protein. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). Generally, it does not interfere with folding or bioactivity. Thus, there is no need for removing the tag. Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. The proteins obtained are bioactive and display a very high purity (above 99%). FEACTURES 1. High capability of antibody binding. 2. Time-saving, simple and mild. 3. Obtained high-purity protein by one-Step. 4. No need removing the tag. SPECIFICATIONS Bead Diameter (µm) - 30μm～150μm Ligand density - ～6mg Strep-Tactin /mL Gel Protein Binding Capacity - ～7mg Strep-tag II protein/mL Gel Slurry Concentration - 10%(v/v) Suspension Storage solution - 1×PBS(0.1%Tween-20+0.05%NaN3) Binding/Washing Buffer - 10mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH8.0 Elution Buffer - 2.5mM desthiobiotin in Binding Buffer Regeneration Buffer - 0.5M NaOH or 1mM HABA