Colorectal cancer assay kit

for BRAF mutationscellreal-time

24 tests ‘kit. Real-time fluorescent quantitative PCR instruments including Stratagene Mx3000P, ABI 7500, Roche LC480, Abbott m2000rt, and BioRad CFX-96. Product Description Adopting the ARMS-PCR method, this kit can be used to detect V600E point mutation in codon 600 of exon 15 of the BRAF gene; BRAF V600E mutation accounts for 80-90% of BRAF mutations. This kit is used for qualitative assay of BRAF V600E point mutation in patients with non-small cell tung cancer (NSCLC), colorectal cancer, andthyroid cancer. The product contains our proprietary stabilizer with which high specificity can be guaranteed and false positives can be avoided under the situation when the sensitivity reaches 1%. The assay result can be used as the reference for clinicians’ selection of population who may receive iressa (gefitinib), tarceva (erlotinib), and conmana (icotinib) among NSCLC patients, population who may receive erbitux (cetuximab) and vectibix (panitumumab) among patients with colorectal cancer, and population who may receive zelboraf among patients with thyroid cancer. The product can be used in in vitro tests only. The assay result can only be used for clinical reference and shall not be used as the only basis for diagnosis and treatment. Product Features High selectivity——Detect as low as 1% of mutations in 5 ng‘ul DNA from multiple types of samples High sensitivity——Only a few hundred copies are needed to detect the target gene High specificity The amplification curve is flattening when wild type samples are tested Easy to operate—— Pre-assembled to facilitate standard operation in the lab Convenient and fast——Suitable for various PCR instruments, test completed in 90 minutes