COVID-19 test kit

SARS-COV-2nasopharyngealRT-qPCR

qualitative detection of 2019 virus RNA in upper respiratory tract specimens (nasopharyngeal and oropharyngeal extracts) by real time PCR systems. It is considered as an aid in the diagnosis of the 2019-virus infection. 2. Principle of Real-Time RT-PCR The principle of the real-time detection is based on the fluorogenic 5'nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5' end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.