Loading buffer reagent SmartCheck

dyeDNA ladderfor DNA extraction

SmartCheck ladders are available in 3 size ranges – 50bp, 100bp and 1kb – to cover most common applications. Ready-to-use formulation includes loading buffer and tracking dye 500µl suitable for 100 lanes (5µl per lane) Higher intensity reference bands Ultra pure production allows economical ambient shipping Technical Details Protocol: Briefly vortex the tube and use 5µl per lane. Additional loading buffer is not required. Concentration: 0.1 mg/ml Tracking dye: bromophenol blue and xylene cyanol Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months. Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality. Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.