Alcohol reagent kit ALC-10 series

solutionfor biochemistrydiagnostic

For the quantitative determination of ethanol in serum and plasma. For in vitro diagnostic use only. METHOD HISTORY Enzymatic UV test with alcohol dehydrogenase (ADH) PRINCIPLE ADH Ethanol + NAD+ Acetaldehyde + NADH + H+ Alcohol dehydrogenase (ADH) catalyzes the oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD+ to NADH. The measured absorbance of the produced NADH is proportional to the ethanol concentartion in the sample REAGENT COMPOSITION R1: Buffer ( pH 9,0) 300 mmol/L R2: Buffer (pH 6,6) 40 mmol/L NAD ≥ 10 mmol/L Alcohol dehydrogenase ≥ 200 kU/L REAGENT PREPARATION Ready to use REAGENT STORAGE AND STABILITY 1.The reagents are stable up to the end of hte indicated month of expiry,protected from light and contamination is avoided. 2.Do not freze the reagents. 3.The standards are stable up to the end of hte indicated month of expiry. 4.The standard bottles must be used immediately after opening since the indicated concentration changes due to evaporation. WARNING AND PRECAUTIONS 1.Reagent 1: Warning. H315 Causes skin irritation. H319 Causes serious eye irritation. P264 Wash hands and face thoroughly after handling. P 280 Wear protective gloves/protective clothing/eye protection/face protection. P302+P352 If on skin:Wash with plenty of water/soap. P305+P351+P338 If in eyes:Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.