Amylase reagent kit AMY-10 series

solutionpreservativediagnostic

For the quantitative kinetic determination of amylase activity in human serum using a manual or automated procedure. PRINCIPLE α -Amylase hydrolyzes the substrate (Gal-G2- α -CNP) 2-chloro-4-nitrophenyl-L-O- α -Dgalactopyranosyl maltoside to release directly 2-chloro -p-nitrophenol (CNP),which is measured colorimetrical-ly at 405 nm. The rate of increase in absorbance is proportional to α -amylase activity in the sample. REAGENT DETERIORATION Do not use if: 1. The absorbance of the working reagent is greater than 0.600 when measured at 405 nm against water in a cuvette with a 1 cm path length. 2. The reagent fails to meet stated parameters of performance. 3. The reagent is turbid or displays other evidence of bacterial contamination. PRECAUTIONS 1. This reagent kit is intended for in vitro diagnostic use only. 2. This reagent contains potassium thiocyanate Avoid ingestion. 3. Reagents are acidic solutions, flush with water when contact occurs. 4. This reagent contains sodium azide (0.01%) as preservative. Do not ingest. May react with lead and copper plumbing to form highly explosive metal azides. Upon disposal, flush with a large volume of water to prevent azide build up. Reagent 1 and Reagent 2 contains Sodium hydroxide . R36/38 : Irritation to eyes and skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective cloth gloves and eye/face protection. SPECIMEN COLLECTION AND HANDLING 1. Unhemolyzed serum is the specimen of choice. 2. Anticoagulants, such as Citrate and EDTA, bind calcium that is needed for amylase activity.