Calcium reagent kit CAL-10 series

solutionpreservativefor biochemistry

For the in vitro quantitative determination of Calcium in serum. METHODOLOGY : This method uses Arsenazo III and has been modified to provide a highly sensitive and stable reagent system . Calcium reacts with Arsenazo III in a slightly alkaline medium to form a purple-colored complex which absorbs at 650 nm. The intensity of the color is proportional to the calcium concentration. Precautions 1. Reagent is for ”in vitro” diagnostic use only. 2. Reagent may be irritating to the skin. Avoid contact. Flush with water if contact occurs. 3. Reagent contains Sodium Azide as a preservative. Reagent contains Imidazole. REAGENT PREPARATION Reagent is supplied ready to use REAGENT DETERİORATİON The reagent should not be used if: 1. The reagent is turbid. 2. The reagent fails to meet stated parameters of performance. SPECIMEN COLLECTION AND STORAGE 1. Fresh, unhemolyzed serum is the preferred specimen. 2. Anticoagulants other than heparin should not be used. 3. Remove serum from clot as soon as possible since red cells can absorb calcium. 4. Older serum specimens containing visible precipitate should not be used. INTERFERENCES 1. Hemoglobin: No significant interference (<5%) from hemoglobin up to 600 mg/dL. Bilirubin: No significant interference (<5%) from bilirubin up to 15 mg/dL. Lipemia: No significant interference (<5%) from lipemia up to 278 mg/dL measured as triglycerides.