Chloride reagent kit CHL-101 series

solutionfor biochemistrynitrate

For the quantitative determination of Chloride in human serum and plasma The intensity of the color formed is proportional to the chloride ion concentration in the sample. REAGENT COMPOSITION Reagent Mercuric thiocyanate 4 mM Ferric nitrate 40 mM Mercuric nitrate 2 mM Nitric acid 45 mM Standard SCHL-103 1x2 mL REAGENT PREPARATION All the reagents are ready to use. Precautions: Corrosive:Causes severe burns. Mercury(II) thiocyanate :Harmful by inhalation, in contact with skin and if swallowed. Keep away from food, drink and animal feeding stuffs. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). This material and its container must be disposed of as hazardous. Avoid release to the environment. REAGENT DETERIORATION -Presence of particles and turbidity. - Blank absorbance (A) at 480 nm ≥ 0,15 SPECIMEN COLLECTION AND STORAGE 1.Serum, plasma ,CSF,sweat. Free of hemolysis and separated from cells as rapidly as possible. Anticoagulants such as oxalate or EDTA are not acceptable they will interfere with results. 2.Urine: Collect 24 hour urine specimen in chloride free containers. Dilute a sample ½ in distilled water. Mix.Multiply results by 2 (dilution factor). INTERFERENCES The following analytes were tested up to the following levels and were found not to interfere : Bovine serum albumin 150 g/L, Bilirubin 120mg/L, Triglycerides 6 g/L, Hemolysis. Anticoagulants other than heparin