Creatinine reagent kit CRE-10 series

solutionfor biochemistryliquid

For the in vitro quantitative determination of Creatinine in serum and urine METHODOLOGY In the first reaction,creatinase and sarcosine oxidase were used in the enzymatic hydrolysis of endogenous creatine to produce hydrogen peroxide ,that is eliminated by catalase .In the second reaction ,the catalase is inhibited by sodium azide and creatinase and 4- aminoantpyrine (4-AAP) were added,and only the creatine generated from creatinine by creatininase was hydrolyzed sequentially by creatinase and sarcosine oxidase to produce hydrogen peroxide.This newly-formed hydrogen peroxide was measured in a coupled reaction catalyzed by peroxidase,with N-ethyl-nsulphopropyl-m-toluidine (TOPS)/4-AAP as a chromogen. REAGENT PREPARATION Reagents are supplied in a two vial, ready to use, liquid form. PRECAUTIONS Reagent 1 contains MOPS ; R36/37/38 Irritation to eyes,respiratory system and skin. Keep locked up ,Keep out of the reach of children. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective cloth gloves and eye/face protection. REAGENT DETERIORATION The reagent should not be used if: 1. The reagent is cloudy (contaminated). 2. The reagent fails to meet stated parameters of performance. INTERFERENCES 1. Studies to determine the level of interference for hemoglobin and bilirubin, were carried out, the following results were obtained: Hemoglobin: No significant interference from hemoglobin up to 5 g/dL. Bilirubin: No significant interference from bilirubin up to 40 mg/dL. 2. Some drugs and substances may interfere accuracy of the creatinine.