Triglycerides reagent kit TRI-10 series

preservativefor biochemistrydiagnostic

For the in vitro quantitative determination of Triglycerides in serum. METHODOLOGY Principle: Triglycerides in the sample are hydrolyzed by lipase to glycerol and fatty acids. The glycerol is then phosphorylated by adenosine-5-triphosphate (ATP) to glycerol-3-phosphate (G3P) and adenosine-5-diphosphate in a reaction catalyzed by glycerol kinase (GK). Glycerol-3-phosphate is then converted to dihydroxyacetone phosphate (DAP) and hydrogen peroxide by glycerophosphate oxidase (GPO). The hydrogen peroxide then reacts with 4-aminophenazone (4-AP) and p-chlorophenol in a reaction catalyzed by peroxidase to yield a red colored quinoneimine dye. The intensity of the color produced is directly proportional to the concentration of Triglycerides in the sample when measured at 505 nm Precautions 1. Reagent contains Sodium Azide as a preservative. 2. This reagent is for in vitro diagnostic use only. REAGENT DETERIORATION DO NOT USE THE REAGENT IF: 1. Reagent is turbid. 2. The reagent has an absorbance greater than 0.400 against water at 505 nm. 3. The reagent fails to recover stated values in control sera. INTERFERENCES No interferences were observed with bilirubin up to 170 µmol/L and hemoglobin up to 10 g/L. ADDITIONAL EQUIPMENT REQUIRED BUT NOT PROVIDED 1. A clinical chemistry analyzer capable maintaining constant temperature (37°C), and measuring absorbance at 490 – 550 nm. 2. Deionized water and related equipment, e.g.: pipettes 3. Analyzer specific consumables, e.g.: sample and read cups 4. Control and calibrator materials. PROCEDURE Wavelength : 505 nm(490-550) Working temperature : 37°C Optical path : 1 cm Assay type : Endpoint