Total proteins reagent kit PRO-10 series

solutionfor biochemistrydiagnostic

For the in vitro quantitative determination of total protein concentration in serum. METHODOLOGY The color reaction of protein molecules with cupric ions, known as the Biuret color reaction, has been known since 1878. Since the Riegler publicatlons of 1914, several attempts have been made to stabilize the cupric ions in the alkali reagent. Kingsley, modified the procedure In 1939 and 1942 to include the use of sodium potassium tartrate as a complexing agent. This procedure was later modified by Weichselbaum and Gornall. The present method Is based on these modifications PRECAUTIONS 1. This reagent is for in vitro diagnostic use only. 2. Avoid ingestion. DO NOT PIPETTE BY MOUTH. In case of ingestion drink large amounts of water and seek medical attention quickly. 3. Avoid contact with skin and eyes. The reagent contains sodium hydroxide which is corrosive. In case of contact with skin, flush with water. For eyes, seek medical attention. Reagent contains Sodium hydroxide . R34 Causes burns Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell seek medical advice immediately (show the label where possible). ADDITIONAL EQUIPMENT REQUIRED BUT NOT PROVIDED: 1. A clinical chemistry analyzer capable maintaining constant temperature (37°C), and measuring absorbance at 540nm. 2. Iron-free deionized water and related equipment, e.g.: pipettes 3. Analyzer specific consumables, e.g.: sample and read cups 4. Control and calibrator materials PROCEDURE Wavelength : 540 nm Working temperature : 37°C Optical path : 1 cm Assay type : Endpoint Direction : Increasing