Potassium reagent kit POT-10 series

solutiondiagnosticlaboratory

For the quantitative determination of Potassium in human serum and plasma. METHODOLOGY Potassium is determined spectrophotometrically through a kinetic coupling assay system using potassium dependent pyruvate kinase. Pyruvate generated is converted to lactate accompanying conversion of NADH analog to NAD analog. The corresponding decrease of optical density at 380nm is proportional to the potassium concentration in the serum. Precautions: 1. This reagent is for in vitro diagnostic use only. 2. DO NOT INGEST. Avoid contact with skin and eyes. 3. These reagents contain lithium azide. Azide compounds may react with lead or copper plumbing to form potentially explosive compounds. Flush drains with co REAGENT DETERIORATION Do not use the reagent if: 1. Presence of particles and turbidity. SPECIMEN COLLECTION AND STORAGE For use non-hemolysed serum . Stability: Serum samples should be collected such that testing is performed as soon as possible and within 5 days after the specimen collection. NTERFERANCES The assay is not interfered by the following substances at indicated concentrations: Na+ 150 mM, NH4 + 0,5 mM, Ca2+7,5 mM, Pi 2.0 mM, ascorbic acid 10,0 mM, Zn2+0.5 mM, Fe3+ 0.5 mM, Cu2+ 0.5 mM, triglycerides 1000 mg/dL, hemoglobin 500 mg/dL, conjugated bilirubin 20 mg/dL and unconjugated bilirubin 15 mg/dL Materials Required but not Provided 1. Spectrophotometer or colorimeter measuring at 380-405 nm. 2. Matched cuvettes 1.0 cm light path. 3. General laboratory equipment. PROCEDURE Wavelength : 380 nm Assay Type : Fixed time Reaction Direction : Decreasing Temperature : 37°C Opthical path : 1 cm