ASO reagent kit ASO – 30050

enzymediagnostictissue

SLO is a toxic immunogenic exoenzyme produced by -heamolitic Streptococci of groups A, C and G. Measuring the ASO antibodies are useful for the diagnostic of rheumatoid fever, acute glomerulonephritis and streptococcal infections. Rheumatic fever is an inflammatory disease affecting connective tissue from several parts of human body as skin, heart, joints etc… and acute glomerulonephritis is a renal infection that affects mainly to renal glommerulus. Principle: The ASO-Turbilatex is a quantitative turbidimetric test for the measurement of ASO in human serum or plasma. Latex particles coated with streptolysin O (SLO) are agglutinated when mixed with samples containing ASO. The agglutination causes an absorbance change, dependent upon the ASO contents of the patient sample that can be quantified by comparison from a calibrator of known ASO concentration PRECAUTIONS Components from human origin have been tested and found to be negative for the presence of HBsAg, HCV, and antibody to HIV (1/2). However handle cautiously as potentially infectious. CALIBRATION bt products recommends using ASOCAL-30 calibrator . Recalibrate when control results are out of specified tolerances, when using different lot of reagent and when the instrument is adjusted. REAGENT PREPARATION Reagents are supplied in a two vial, ready to use, liquid form. Swirl the latex vial gently before use. For some analyzers, the reagents can be combined to make a working solution by mixing 4 part of Diluent (R1) with 1 part of Latex (R2) (e.g., 4mL R1 + 1mL R2).